Composite

Part:BBa_K4205006:Design

Designed by: Yuq iHuang   Group: iGEM22_NJMU-China   (2022-09-10)


PnisA-nisRK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we add two restriction sites at both ends of this fragment in order to insert the fragment into our vector plasmid PLDHLH673.


Source

we got the sequence of PnisA and nisRK from igem parts, and find a company to synthesize them.

References